منابع مشابه
Nucleoskeleton and initiation of DNA replication in metazoan cells.
To determine whether or not initiation sites for DNA replication in mammalian cells are defined by association with nuclear structure, attachments between the nucleoskeleton and the hamster DHFR gene initiation zone were examined. Nucleoskeletons were prepared by encapsulating cells in agarose and then extracting them with a nonionic detergent in a physiological buffer. The fraction of DNA that...
متن کاملThe nucleoskeleton: active site of transcription and replication.
Nuclei and chromatin are rarely studied at a physiological salt concentration since they aggregate so readily [16). As a result, they are generally studied in the presence of "stabilizing" divalent cations under hyperor hypotonic conditions. Such conditions are unsatisfactory for several reasons. The "stabilizing" cations activate nucleases, destroying template integrity and supercoiling, and u...
متن کاملMutational bias suggests that replication termination occurs near the dif site, not at Ter sites.
In bacteria, Ter sites bound to Tus/Rtp proteins halt replication forks moving only in one direction, providing a convenient mechanism to terminate them once the chromosome had been replicated. Considering the importance of replication termination and its position as a checkpoint in cell division, the accumulated knowledge on these systems has not dispelled fundamental questions regarding its r...
متن کاملBreak-induced replication occurs by conservative DNA synthesis.
Break-induced replication (BIR) refers to recombination-dependent DNA synthesis initiated from one end of a DNA double-strand break and can extend for more than 100 kb. BIR initiates by Rad51-catalyzed strand invasion, but the mechanism for DNA synthesis is not known. Here, we used BrdU incorporation to track DNA synthesis during BIR and found that the newly synthesized strands segregate with t...
متن کاملGrapevine fanleaf virus replication occurs on endoplasmic reticulum-derived membranes.
Infection by Grapevine fanleaf nepovirus (GFLV), a bipartite RNA virus of positive polarity belonging to the Comoviridae family, causes extensive cytopathic modifications of the host endomembrane system that eventually culminate in the formation of a perinuclear "viral compartment." We identified by immunoconfocal microscopy this compartment as the site of virus replication since it contained t...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1986
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1986.tb04374.x